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CAS 2382-43-6 Methacholine Reference Standard Methacholine Reference Standard High Purity USP Grade Reference Standard

Beta Methyl Choline Chloride

IUPAC: 2-Hydroxy-N,N,N-trimethylpropan-1-aminium chloride View on PubChem
CAS NUMBER
2382-43-6
MOL. FORMULA
C6H16ClNO
MOL. WEIGHT
153.65 g/mol
Scientific Overview

As a quaternary ammonium salt closely related to acetylcholine, beta-methylcholine chloride's structure underlies its use as the reference/identity standard for methacholine chloride quality control. Methacholine itself acts as a direct-acting muscarinic receptor agonist administered via inhalation challenge testing to provoke controlled, reversible bronchoconstriction, helping clinicians assess airway responsiveness in patients with suspected asthma.

Beta-Methylcholine Chloride (2-hydroxypropyltrimethylammonium chloride) is a quaternary ammonium compound structurally related to choline, bearing a methyl substituent at the beta carbon. It is officially designated as the USP reference standard for Methacholine Chloride, the muscarinic agonist used in bronchial provocation testing for diagnosing asthma and airway hyperreactivity.

Full Technical Data
Chemical Name Beta-Methylcholine Chloride (2-Hydroxypropyltrimethylammonium Chloride)
CAS Number 2382-43-6
IUPAC Name 2-Hydroxy-N,N,N-trimethylpropan-1-aminium chloride
Molecular Formula C6H16ClNO
Molecular Weight 153.65 g/mol g/mol
Reaction Scheme
Propylene Oxide
Trimethylamine
NA
Beta-Methylcholine Chloride
NA
Commercial Applications

Methacholine Chloride Reference Standard

USP-designated identity/quality control standard for methacholine chloride bronchial challenge test formulations.

Pulmonary Diagnostic Testing Support

Underpins analytical method validation for methacholine challenge test kits used in asthma diagnosis.

Cholinergic Pharmacology Research

Used as a structural reference in muscarinic receptor agonist research and quaternary ammonium compound studies.

Anion Exchange Membrane Research

Applied in preparing chitosan-quaternized derivatives for anion exchange membrane fuel cell studies.

Market Therapeutic Focus
METHACHOLINE REFERENCE STANDARD PHARMACEUTICAL MANUFACTURING RESPIRATORY DIAGNOSTICS (METHACHOLINE CHALLENGE) ANALYTICAL REFERENCE STANDARDS CHOLINERGIC PHARMACOLOGY R&D CRO/CDMO
Scientific Literature

Methacholine Challenge Testing: Guidelines and Interpretation

Authors: Crapo, R.O. et al.

Am. J. Respir. Crit. Care Med.

Structure-Activity Relationships of Choline Ester Muscarinic Agonists

Authors: Barlow, R.B. et al.

Br. J. Pharmacol.

Quaternary Ammonium Compounds in Anion Exchange Membrane Applications

Authors: Merle, G. et al.

J. Membr. Sci.

Peer Queries & FAQs
What is the relationship between this compound and methacholine chloride?
Beta-methylcholine chloride is chemically equivalent to methacholine chloride; it serves as the official USP reference/identity standard used for quality control and method validation of methacholine chloride drug products.
What is methacholine challenge testing used for?
It's a diagnostic procedure administering inhaled methacholine to provoke controlled bronchoconstriction, helping identify airway hyperresponsiveness characteristic of asthma.
Why must this compound be handled carefully in storage?
It is hygroscopic, meaning it readily absorbs atmospheric moisture, which can affect purity and stability if not stored in a tightly sealed, moisture-protected container.
What is the recommended storage condition?
Store in a tightly sealed container at room temperature, protected from moisture (hygroscopic material).

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