Originally developed as a modification of Eagle's Minimum Essential Medium with increased amino acid and vitamin concentrations, DMEM High Glucose provides an elevated glycolytic energy source that supports the high metabolic demands of many immortalized and transformed cell lines. It is typically supplemented with fetal bovine serum, L-glutamine, and antibiotics before use.
DMEM High Glucose Medium is a widely used basal cell culture medium containing 4.5 g/L D-glucose along with amino acids, vitamins, inorganic salts, and other nutrients, formulated to support the growth of a broad range of adherent and suspension mammalian cell lines.
| Chemical Name | Dulbecco's Modified Eagle Medium (DMEM), High Glucose |
| CAS Number | N/A (formulated mixture) |
| IUPAC Name | N/A (defined multi-component formulation) |
| Molecular Formula | N/A (formulated mixture) |
| Molecular Weight | D-Glucose:180.16 g/mol, g/mol |
| Boiling Point | na |
| Density | na |
| Refractive Index | na |
| Flash Point | na |
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