Home / Products / DMEM High Glucose Medium
CAS N/A (formulated mixture) Cell Culture Basal Medium Cell Culture Basal Medium

DMEM High Glucose Medium

IUPAC: N/A (defined multi-component formulation)
CAS NUMBER
N/A (formulated mixture)
MOL. FORMULA
N/A (formulated mixture)
MOL. WEIGHT
N/A
Get Commercial Quote Request Sample Request COA
Scientific Overview

Originally developed as a modification of Eagle's Minimum Essential Medium with increased amino acid and vitamin concentrations, DMEM High Glucose provides an elevated glycolytic energy source that supports the high metabolic demands of many immortalized and transformed cell lines. It is typically supplemented with fetal bovine serum, L-glutamine, and antibiotics before use.

DMEM High Glucose Medium is a widely used basal cell culture medium containing 4.5 g/L D-glucose along with amino acids, vitamins, inorganic salts, and other nutrients, formulated to support the growth of a broad range of adherent and suspension mammalian cell lines.

Full Technical Data
Chemical Name Dulbecco's Modified Eagle Medium (DMEM), High Glucose
CAS Number N/A (formulated mixture)
IUPAC Name N/A (defined multi-component formulation)
Molecular Formula N/A (formulated mixture)
Molecular Weight N/A g/mol
Reaction Scheme
Component Amino Acids, Vitamins, Inorganic Salts, D-Glucose
NA
NA
DMEM High Glucose Medium
NA
Commercial Applications

Mammalian Cell Culture

Basal growth medium for a wide range of adherent cell lines (e.g. HEK293, HeLa, CHO) in research and bioprocessing.

Recombinant Protein & Antibody Production

Base medium for upstream cell culture in biologics and monoclonal antibody manufacturing workflows.

Vaccine Production Substrate Culture

Used to culture mammalian cell substrates for viral vaccine antigen production.

Cell Therapy & Regenerative Medicine

Supports expansion of stem cells and other cell types used in cell-therapy manufacturing.

Market Therapeutic Focus
CELL CULTURE BASAL MEDIUM PHARMACEUTICAL MANUFACTURING CELL & GENE THERAPY / BIOPROCESSING RECOMBINANT PROTEIN PRODUCTION ACADEMIC & INDUSTRIAL RESEARCH CRO/CDMO
Scientific Literature

Studies of a Tumor Producing Line of Chick Sarcoma Cells: I. Cultivation in Suspension

Authors: Dulbecco, R.; Freeman, G.

Virology

DOI: 10.1016/0042-6822(59)90048-3

A Modification of Eagle's Medium Increasing the Growth of Mouse Embryo Cells

Authors: Smith, J.D. et al.

Exp. Cell Res.

DOI: 10.1016/0014-4827(60)90040-2

Cell Culture Media: A Review

Authors: Yao, T.; Asayama, Y.

Reprod. Med. Biol.

DOI: 10.1002/reg2.91
Peer Queries & FAQs
What does 'High Glucose' mean in DMEM's name?
It refers to a D-glucose concentration of 4.5 g/L, versus the 'low glucose' DMEM formulation at 1.0 g/L; the higher glucose level supports faster-growing or more metabolically demanding cell lines.
Does DMEM contain serum or antibiotics?
No, base DMEM is a defined basal formulation; serum (commonly FBS), L-glutamine, and antibiotics are added separately by the end user according to their specific cell culture protocol.
What cell types is DMEM High Glucose best suited for?
It is broadly used for many adherent immortalized and transformed cell lines, including HEK293, HeLa, CHO, and various fibroblast lines.
What is the recommended storage condition?
Store liquid medium at 2-8°C protected from light; use within the manufacturer's stated shelf life and avoid repeated freeze-thaw if frozen aliquots are used.

Need a Custom Derivative or Labeled Analog?

Our R&D centers specialize in the design and process development of complex heterocyclic and chiral molecules — mg to kg scale.

Request Custom Synthesis Quote Check Manufacturing Scale