IPTG functions by binding to the lac repressor (LacI) protein, inducing a conformational change that releases the repressor from the operator DNA and permits transcription of genes under lac promoter/operator control. Because it cannot be metabolized by ß-galactosidase, IPTG concentration remains constant throughout induction, ensuring stable and reproducible expression kinetics. Removal of dioxane is critical for applications where solvent traces could compromise cell viability, protein folding, or downstream purification.
IPTG (Isopropyl ß-D-1-thiogalactopyranoside) is a non-metabolizable synthetic analog of allolactose used as a potent inducer of the lac operon in E. coli expression systems. The dioxane-free grade eliminates residual 1,4-dioxane, a solvent byproduct from standard synthesis routes, making it suitable for sensitive cell culture and downstream biopharmaceutical applications.
| Chemical Name | Isopropyl ß-D-1-thiogalactopyranoside (IPTG), Dioxane-Free |
| CAS Number | 367-93-1 |
| IUPAC Name | Propan-2-yl 1-thio-ß-D-galactopyranoside |
| Molecular Formula | C9H18O5S |
| Molecular Weight | 238.30 g/mol g/mol |
Standard inducer for IPTG-inducible promoter systems (e.g., T7, lac, tac) in E. coli protein production.
Used alongside X-Gal/Bluo-Gal to identify recombinant clones via ß-galactosidase induction.
Dioxane-free grade preferred for GMP-adjacent bioprocessing where solvent purity affects cell health and product quality.
Widely used tool for studying lac operon regulation and inducible gene expression systems.
J. Ind. Microbiol.
Protein Expr. Purif.
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