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CAS 56-85-9 Cell Culture Medium Supplement (Amino Acid) Cell Culture Medium Supplement (Amino Acid)

L-Glutamine Solution 200 mM

IUPAC: (2S)-2,5-Diamino-5-oxopentanoic acid View on PubChem
CAS NUMBER
56-85-9
MOL. FORMULA
C5H10N2O3
MOL. WEIGHT
146.15 g/mol
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Scientific Overview

L-Glutamine is consumed rapidly by cultured cells as both an energy substrate (via glutaminolysis) and a precursor for nucleotide and protein biosynthesis, but it is thermally and hydrolytically unstable in aqueous solution, spontaneously degrading to ammonia and pyrrolidone carboxylic acid over time. It is therefore supplied as a concentrated frozen stock solution added fresh to base media just before use, rather than being included in the shelf-stable powdered medium formulation.

L-Glutamine Solution 200 mM is a sterile-filtered, ready-to-use cell culture supplement providing the essential amino acid L-glutamine, a major energy source and nitrogen donor required for growth of most mammalian cell lines in vitro.

Full Technical Data
Chemical Name L-Glutamine Solution, 200 mM
CAS Number 56-85-9
IUPAC Name (2S)-2,5-Diamino-5-oxopentanoic acid
Molecular Formula C5H10N2O3
Molecular Weight 146.15 g/mol g/mol
Reaction Scheme
Fermentation-derived L-Glutamic Acid (or Sugar Beet-derived fermentation)
Ammonia
NA
L-Glutamine Solution 200 mM
NA
Commercial Applications

Cell Culture Medium Supplementation

Standard supplement added to DMEM, RPMI, and other basal media to support cell growth and viability.

Recombinant Protein & Antibody Production

Supports the high metabolic and biosynthetic demands of cell lines used in biologics manufacturing.

Cell & Gene Therapy Manufacturing

Essential nutrient supplement in cell expansion protocols for cell-therapy product manufacturing.

Metabolic & Cancer Cell Biology Research

Used to study glutaminolysis and amino-acid-dependent metabolic pathways in cultured cells.

Market Therapeutic Focus
CELL CULTURE MEDIUM SUPPLEMENT (AMINO ACID) PHARMACEUTICAL MANUFACTURING CELL & GENE THERAPY / BIOPROCESSING RECOMBINANT PROTEIN PRODUCTION ACADEMIC & INDUSTRIAL RESEARCH CRO/CDMO
Scientific Literature

Glutamine Metabolism in Cancer: Understanding the Heterogeneity

Authors: Yoo, H.C. et al.

Exp. Mol. Med.

DOI: 10.1038/s12276-020-00504-8

Industrial Production of L-Glutamine

Authors: Kusumoto, I.

J. Nutr.

DOI: 10.1093/jn/131.9.2552S

Glutamine and Cell Signaling in Cancer

Authors: Cluntun, A.A. et al.

Cell Metab.

DOI: 10.1016/j.cmet.2017.01.008
Peer Queries & FAQs
Why is L-Glutamine added as a separate solution instead of being in the base medium powder?
It degrades relatively quickly in aqueous solution (breaking down into ammonia and pyroglutamate), so manufacturers supply it as a stable frozen concentrate to be added fresh rather than including it in shelf-stable liquid or powdered media.
What concentration is typically used in complete culture medium?
A 200 mM stock is diluted to a final working concentration of approximately 2-4 mM in most standard mammalian cell culture media formulations.
Can GlutaMAX or similar dipeptide forms replace L-Glutamine?
Yes, stabilized dipeptide forms (e.g., L-alanyl-L-glutamine) are increasingly used in modern serum-free media because they resist spontaneous degradation better than free L-glutamine.
What is the recommended storage condition?
Store frozen at -10 to -30°C; once thawed, use within approximately 2 weeks at 4°C and avoid repeated freeze-thaw cycles.

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